Changes in Potential Pathogens and Antibiotic Resistance-Related Functional Profiles during the Bioconversion of Chicken Manure by Black Soldier Fly Larvae

Authors

  • Xueyi Ren College of Engineering and Technology, Tianjin Agricultural University, Tianjin, 300392, China
  • Shuai Han College of Engineering and Technology, Tianjin Agricultural University, Tianjin, 300392, China
  • Xinyu Geng College of Engineering and Technology, Tianjin Agricultural University, Tianjin, 300392, China
  • Baolu Wang College of Engineering and Technology, Tianjin Agricultural University, Tianjin, 300392, China
  • Nan Wu College of Engineering and Technology, Tianjin Agricultural University, Tianjin, 300392, China

DOI:

https://doi.org/10.54691/p6rbf718

Keywords:

Black Soldier Fly; Antibiotic Resistance; Pathogenic Bacteria; Secondary Composting; High-Throughput Sequencing.

Abstract

Black soldier fly larvae (BSFL) bioconversion is considered a promising approach for the harmless treatment and resource utilization of livestock manure. In this study, chicken manure and its residues after BSFL bioconversion and secondary composting were investigated to evaluate changes in potential pathogens and antibiotic resistance-related functions using high-throughput sequencing-based analysis. A total of 12 potential pathogenic species were detected throughout the treatment process. Chicken manure (CM) showed the highest abundance of potential pathogens, with Escherichia coli being dominant. Its relative abundance decreased from 0.2166 in CM to 0.0001 after secondary composting, and the other potential pathogens also exhibited an overall declining trend. Analysis of antibiotic resistance-related functional profiles showed differential responses among functional categories. The relative abundance of vancomycin resistance-related functions decreased steadily from approximately 0.280% to 0.220%, whereas β-lactam resistance-related functions changed little after BSFL bioconversion and increased to approximately 0.450% after secondary composting. These results indicate that BSFL bioconversion combined with secondary composting can effectively reduce some potential pathogens and certain antibiotic resistance-related functions in chicken manure, but the responses of different resistance types vary. Further attention should therefore be paid to the persistence and potential dissemination risk of specific resistance traits during manure resource utilization.

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References

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Published

2026-10-21

Issue

Vol. 8 No. 10 (2026)

Section

Articles

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